An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays

Thomas Reinard; Hans Jörg Jacobsen

doi:10.1016/0003-2697(89)90286-8

An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays

Thomas Reinard^*
, Hans Jörg Jacobsen

^*Korrespondierende*r Autor*in für diese Arbeit

Rheinische Friedrich-Wilhelms-Universität Bonn

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Abstract

A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.

Originalsprache	Englisch
Seiten (von - bis)	157-160
Seitenumfang	4
Fachzeitschrift	Analytical biochemistry
Jahrgang	176
Ausgabenummer	1
DOIs	https://doi.org/10.1016/0003-2697(89)90286-8
Publikationsstatus	Veröffentlicht - Jan. 1989
Extern publiziert	Ja

ASJC Scopus Sachgebiete

Biophysik
Biochemie
Molekularbiologie
Zellbiologie

Zugriff auf Dokument

10.1016/0003-2697(89)90286-8

Andere Dateien und Links

Verknüpfung zur Publikation in Scopus

Dieses zitieren

@article{9de5b2cdcabe41ee848db3c9175d7896,

title = "An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays",

abstract = "A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.",

author = "Thomas Reinard and Jacobsen, \{Hans J{\"o}rg\}",

year = "1989",

month = jan,

doi = "10.1016/0003-2697(89)90286-8",

language = "English",

volume = "176",

pages = "157--160",

journal = "Analytical biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays

AU - Reinard, Thomas

AU - Jacobsen, Hans Jörg

PY - 1989/1

Y1 - 1989/1

N2 - A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.

AB - A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.

UR - https://www.scopus.com/pages/publications/0024551980

U2 - 10.1016/0003-2697(89)90286-8

DO - 10.1016/0003-2697(89)90286-8

M3 - Article

C2 - 2712285

AN - SCOPUS:0024551980

SN - 0003-2697

VL - 176

SP - 157

EP - 160

JO - Analytical biochemistry

JF - Analytical biochemistry

IS - 1

ER -