Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Shu Shan Gao; Joanne Hothersall; Ji'En Wu; Annabel C. Murphy; Zhongshu Song; Elton R. Stephens; Christopher M. Thomas; Matthew P. Crump; Russell J. Cox; Thomas J. Simpson; Christine L. Willis

doi:10.1021/ja501731p

Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Shu Shan Gao
, Joanne Hothersall
, Ji'En Wu
, Annabel C. Murphy
, Zhongshu Song
, Elton R. Stephens
, Christopher M. Thomas
, Matthew P. Crump
, Russell J. Cox
, Thomas J. Simpson^*
, Christine L. Willis

^*Korrespondierende*r Autor*in für diese Arbeit

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Abstract

Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

Originalsprache	Englisch
Seiten (von - bis)	5501-5507
Seitenumfang	7
Fachzeitschrift	Journal of the American Chemical Society
Jahrgang	136
Ausgabenummer	14
DOIs	https://doi.org/10.1021/ja501731p
Publikationsstatus	Veröffentlicht - 9 Apr. 2014
Extern publiziert	Ja

ASJC Scopus Sachgebiete

Katalyse
Allgemeine Chemie
Biochemie
Kolloid- und Oberflächenchemie

Zugriff auf Dokument

10.1021/ja501731p

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title = "Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways",

abstract = "Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.",

author = "Gao, \{Shu Shan\} and Joanne Hothersall and Ji'En Wu and Murphy, \{Annabel C.\} and Zhongshu Song and Stephens, \{Elton R.\} and Thomas, \{Christopher M.\} and Crump, \{Matthew P.\} and Cox, \{Russell J.\} and Simpson, \{Thomas J.\} and Willis, \{Christine L.\}",

year = "2014",

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doi = "10.1021/ja501731p",

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AU - Gao, Shu Shan

AU - Hothersall, Joanne

AU - Wu, Ji'En

AU - Murphy, Annabel C.

AU - Song, Zhongshu

AU - Stephens, Elton R.

AU - Thomas, Christopher M.

AU - Crump, Matthew P.

AU - Cox, Russell J.

AU - Simpson, Thomas J.

AU - Willis, Christine L.

PY - 2014/4/9

Y1 - 2014/4/9

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AB - Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

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