Feruloyl esterases from Schizophyllum commune to treat food industry side-streams

Annabel Nieter; Sebastian Kelle; Diana Linke; Ralf G. Berger

doi:10.1016/j.biortech.2016.08.045

Feruloyl esterases from Schizophyllum commune to treat food industry side-streams

Annabel Nieter^*
, Sebastian Kelle
, Diana Linke
, Ralf G. Berger

^*Korrespondierende*r Autor*in für diese Arbeit

Institut für Lebensmittelchemie

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Abstract

Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L⁻¹. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.

Originalsprache	Englisch
Seiten (von - bis)	38-46
Seitenumfang	9
Fachzeitschrift	Bioresource technology
Jahrgang	220
DOIs	https://doi.org/10.1016/j.biortech.2016.08.045
Publikationsstatus	Veröffentlicht - 12 Aug. 2016

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2015 einigten sich die UN-Mitgliedstaaten auf 17 globale Ziele für nachhaltige Entwicklung (Sustainable Development Goals, SDGs) zur Beendigung von Armut, zum Schutz des Planeten und zur Förderung des allgemeinen Wohlstands. Hiermit leisten wir einen Beitrag zu folgendem/n Ziel(en) für nachhaltige Entwicklung (SDGs):

SDG 7 Erschwingliche und saubere Energie

ASJC Scopus Sachgebiete

Bioengineering
Environmental engineering
Erneuerbare Energien, Nachhaltigkeit und Umwelt
Abfallwirtschaft und -entsorgung

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10.1016/j.biortech.2016.08.045

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title = "Feruloyl esterases from Schizophyllum commune to treat food industry side-streams",

abstract = "Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60\%), ferulic (>80\%) and p-coumaric acid (100\%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.",

keywords = "Agro-industrial side-streams, Basidiomycota, Benzoic acid ester, Feruloyl esterase, Pichia pastoris",

author = "Annabel Nieter and Sebastian Kelle and Diana Linke and Berger, \{Ralf G.\}",

note = "Funding information: Support of the work by the Federal Ministry of Education and Research cluster Biokatalyse2021 (P 37) and by M. Bunzel (Department of Food Chemistry and Phytochemistry, KIT, Karlsruhe, Germany) is gratefully acknowledged.",

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doi = "10.1016/j.biortech.2016.08.045",

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AU - Nieter, Annabel

AU - Kelle, Sebastian

AU - Linke, Diana

AU - Berger, Ralf G.

N1 - Funding information: Support of the work by the Federal Ministry of Education and Research cluster Biokatalyse2021 (P 37) and by M. Bunzel (Department of Food Chemistry and Phytochemistry, KIT, Karlsruhe, Germany) is gratefully acknowledged.

PY - 2016/8/12

Y1 - 2016/8/12

N2 - Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.

AB - Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.

KW - Agro-industrial side-streams

KW - Basidiomycota

KW - Benzoic acid ester

KW - Feruloyl esterase

KW - Pichia pastoris

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