TY - JOUR
T1 - Magnetic ZIF-8 as a stable support for biomolecules via adsorption in aqueous buffered solutions at pH = 7
AU - Aguirre, Matías E.
AU - Marino, Ignacio
AU - Bozzano, Patricia B.
AU - Feldhoff, Armin
AU - Grela, María Alejandra
N1 - Funding Information:
This work was financially supported by ANPCyT (ARGENTINA) project 1456 . M.E.A. is a member of the research staff of CONICET. Help of Dr. María Sandra Churio and assistance in TEM investigations by Frank Steinbach are appreciated. The authors also thank Dr. Aurélien Gasnier (Departamento Fisicoquímica de Materiales, Centro Atómico Bariloche, CNEA/CONICET) for technical assistance in the surface area determinations.
PY - 2019/7
Y1 - 2019/7
N2 - Metal-organic frameworks (MOFs) have been recently proposed as supports for biomolecules immobilization, particularly for enzymes. However, their stability under the high ionic strength aqueous solutions required for practical applications of these biocatalysts have not been evaluated. Here, it is shown that surface modifications and a careful analysis and selection of the buffer components must be accomplished to ensure the resistance of the MOF to hydrolysis, even for the previously assumed robust Zeolitic Imidazole Frameworks (ZIFs). By taking into account these considerations, and introducing polyvinylpyrrolidone (PVP) as a surfactant in the synthetic process, a resistant and stable magnetic MOF (Fe 3 O 4 @ZIF-8/PVP) was obtained. Further, the loading capacity of the stabilized synthetized structures for the immobilization of a model protein, such as bovine serum albumin (BSA), was analyzed. It was found that physical interaction provides an efficient and suitable method to immobilize this biomolecule without evidence of leaching.
AB - Metal-organic frameworks (MOFs) have been recently proposed as supports for biomolecules immobilization, particularly for enzymes. However, their stability under the high ionic strength aqueous solutions required for practical applications of these biocatalysts have not been evaluated. Here, it is shown that surface modifications and a careful analysis and selection of the buffer components must be accomplished to ensure the resistance of the MOF to hydrolysis, even for the previously assumed robust Zeolitic Imidazole Frameworks (ZIFs). By taking into account these considerations, and introducing polyvinylpyrrolidone (PVP) as a surfactant in the synthetic process, a resistant and stable magnetic MOF (Fe 3 O 4 @ZIF-8/PVP) was obtained. Further, the loading capacity of the stabilized synthetized structures for the immobilization of a model protein, such as bovine serum albumin (BSA), was analyzed. It was found that physical interaction provides an efficient and suitable method to immobilize this biomolecule without evidence of leaching.
KW - Adsorption
KW - Biomolecule support
KW - Buffered media
KW - Metal-organic frameworks
KW - ZIF-8 stability
UR - https://www.scopus.com/pages/publications/85065846800
U2 - 10.1016/j.inoche.2019.05.013
DO - 10.1016/j.inoche.2019.05.013
M3 - Article
AN - SCOPUS:85065846800
SN - 1387-7003
VL - 105
SP - 225
EP - 229
JO - Inorganic chemistry communications
JF - Inorganic chemistry communications
ER -