Stress response of Nidula niveo-tomentosa to UV-A light

Daniela E. Taupp; Manfred Nimtz; Ralf G. Berger; Holger Zorn

doi:10.3852/07-179R

Stress response of Nidula niveo-tomentosa to UV-A light

Daniela E. Taupp
, Manfred Nimtz
, Ralf G. Berger
, Holger Zorn^*

^*Korrespondierende*r Autor*in für diese Arbeit

Institut für Lebensmittelchemie

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Abstract

Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveo-tomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.

Originalsprache	Englisch
Seiten (von - bis)	529-538
Seitenumfang	10
Fachzeitschrift	MYCOLOGIA
Jahrgang	100
Ausgabenummer	4
DOIs	https://doi.org/10.3852/07-179R
Publikationsstatus	Veröffentlicht - Juli 2008

ASJC Scopus Sachgebiete

Ökologie, Evolution, Verhaltenswissenschaften und Systematik
Physiologie
Molekularbiologie
Genetik
Zellbiologie

Zugriff auf Dokument

10.3852/07-179R

Andere Dateien und Links

Link zur Publikation in Scopus

Dieses zitieren

@article{08ef83f1688d42b6a2406b0e086a1bf1,

title = "Stress response of Nidula niveo-tomentosa to UV-A light",

abstract = "Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveo-tomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.",

keywords = "2-D electrophoresis, Basidiomycete, cDNA, De novo sequencing",

author = "Taupp, \{Daniela E.\} and Manfred Nimtz and Berger, \{Ralf G.\} and Holger Zorn",

year = "2008",

month = jul,

doi = "10.3852/07-179R",

language = "English",

volume = "100",

pages = "529--538",

journal = "MYCOLOGIA",

issn = "0027-5514",

publisher = "Taylor and Francis Ltd.",

number = "4",

}

TY - JOUR

T1 - Stress response of Nidula niveo-tomentosa to UV-A light

AU - Taupp, Daniela E.

AU - Nimtz, Manfred

AU - Berger, Ralf G.

AU - Zorn, Holger

PY - 2008/7

Y1 - 2008/7

N2 - Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveo-tomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.

AB - Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveo-tomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.

KW - 2-D electrophoresis

KW - Basidiomycete

KW - cDNA

KW - De novo sequencing

UR - https://www.scopus.com/pages/publications/54049118783

U2 - 10.3852/07-179R

DO - 10.3852/07-179R

M3 - Article

C2 - 18833746

AN - SCOPUS:54049118783

SN - 0027-5514

VL - 100

SP - 529

EP - 538

JO - MYCOLOGIA

JF - MYCOLOGIA

IS - 4

ER -