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Correlated Raman/FLIM Microscopy System

  • Birgit Stiller (Head) &
  • Michael Steinke (Operator)

Research infrastructure/Equipment: Major Research Instrumentation

    Equipments Details

    Description

    The modular Raman FLIM microscope from Horiba combines Raman analysis and fluorescence lifetime imaging (FLIM) in a single platform for correlated material and structural analysis.
    A standard Olympus BX53 microscope serves as the optical basis. Excitation uses a 532 nm DPSS laser (Oxxius, L1S-532S) for Raman and a 488 nm laser diode (Horiba, DD-485L) for FLIM investigations. Spectral resolution is provided by an iHR-320 spectrometer (Horiba) with 320 mm focal length in combination with a cooled CCD detector (Horiba, Syncerity 1024x256-OE) for Raman and photoluminescence and a single photon counter (Horiba PPD-900) for FLIM. By time-correlated single photon counting (TCSPC), fluorescence lifetimes from picoseconds to seconds can be analyzed.

    Details

    Name5700034172 SMS320-FS-FS-MIL
    Acquisition date26 Jun 2024
    ManufacturersHORIBA Europe GmbH

    DFG Instrumentation Classification Key

    • Optics, Electron Optics / Interferometers, Polarimeters, Refractometers / 5360 Instruments for Scattered and Reflected Light, Optical Surface Testing