A simple light path modifying device to reduce scattering in front-face fluorescence spectra

Frank B. Peters; Andreas Otto Rapp

doi:10.1088/2050-6120/ad5415

A simple light path modifying device to reduce scattering in front-face fluorescence spectra

Frank B. Peters, Andreas Otto Rapp

Institute of Vocational Sciences in the Building Trade

Research output: Contribution to journal › Article › Transfer › peer review

Abstract

This technical note presents a device to diminish scattering signal in front-face fluorescence spectra while obtaining fluorescence signal. The beam path in a commercial fluorescence spectrometer was modified by two deflecting mirrors, leading reflections away from the sensor. This light path modifying (LPM) device was tested with two fluid and three solid substances, where the scattering-to-fluorescence ratio improved by a factor of 1.7 to 7.6. The spectra obtained with the LPM were much clearer, and distortion of the fluorescence peaks was avoided. Scans of quinine sulphate complied well with reference spectra.

Original language	English
Article number	037001
Journal	Methods and Applications in Fluorescence
Volume	12
Issue number	3
DOIs	https://doi.org/10.1088/2050-6120/ad5415
Publication status	Published - 17 Jun 2024

Keywords

device
front-face
fluorescence
scattering
solid sample

ASJC Scopus subject areas

Instrumentation
Atomic and Molecular Physics, and Optics
General Materials Science
Spectroscopy

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10.1088/2050-6120/ad5415Licence: CC BY 4.0

Cite this

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title = "A simple light path modifying device to reduce scattering in front-face fluorescence spectra",

abstract = "This technical note presents a device to diminish scattering signal in front-face fluorescence spectra while obtaining fluorescence signal. The beam path in a commercial fluorescence spectrometer was modified by two deflecting mirrors, leading reflections away from the sensor. This light path modifying (LPM) device was tested with two fluid and three solid substances, where the scattering-to-fluorescence ratio improved by a factor of 1.7 to 7.6. The spectra obtained with the LPM were much clearer, and distortion of the fluorescence peaks was avoided. Scans of quinine sulphate complied well with reference spectra.",

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author = "Peters, {Frank B.} and Rapp, {Andreas Otto}",

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AU - Rapp, Andreas Otto

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N2 - This technical note presents a device to diminish scattering signal in front-face fluorescence spectra while obtaining fluorescence signal. The beam path in a commercial fluorescence spectrometer was modified by two deflecting mirrors, leading reflections away from the sensor. This light path modifying (LPM) device was tested with two fluid and three solid substances, where the scattering-to-fluorescence ratio improved by a factor of 1.7 to 7.6. The spectra obtained with the LPM were much clearer, and distortion of the fluorescence peaks was avoided. Scans of quinine sulphate complied well with reference spectra.

AB - This technical note presents a device to diminish scattering signal in front-face fluorescence spectra while obtaining fluorescence signal. The beam path in a commercial fluorescence spectrometer was modified by two deflecting mirrors, leading reflections away from the sensor. This light path modifying (LPM) device was tested with two fluid and three solid substances, where the scattering-to-fluorescence ratio improved by a factor of 1.7 to 7.6. The spectra obtained with the LPM were much clearer, and distortion of the fluorescence peaks was avoided. Scans of quinine sulphate complied well with reference spectra.

KW - device

KW - front-face

KW - fluorescence

KW - scattering

KW - solid sample

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DO - 10.1088/2050-6120/ad5415

M3 - Article

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VL - 12

JO - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

IS - 3

M1 - 037001

ER -

A simple light path modifying device to reduce scattering in front-face fluorescence spectra

Abstract

Keywords

ASJC Scopus subject areas

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