An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays

Thomas Reinard; Hans Jörg Jacobsen

doi:10.1016/0003-2697(89)90286-8

An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays

Thomas Reinard^*
, Hans Jörg Jacobsen

^*Corresponding author for this work

University of Bonn

Research output: Contribution to journal › Article › Research › peer review

Abstract

A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.

Original language	English
Pages (from-to)	157-160
Number of pages	4
Journal	Analytical biochemistry
Volume	176
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(89)90286-8
Publication status	Published - Jan 1989
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(89)90286-8

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abstract = "A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.",

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An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays

Abstract

ASJC Scopus subject areas

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