Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Shu Shan Gao; Joanne Hothersall; Ji'En Wu; Annabel C. Murphy; Zhongshu Song; Elton R. Stephens; Christopher M. Thomas; Matthew P. Crump; Russell J. Cox; Thomas J. Simpson; Christine L. Willis

doi:10.1021/ja501731p

Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Shu Shan Gao
, Joanne Hothersall
, Ji'En Wu
, Annabel C. Murphy
, Zhongshu Song
, Elton R. Stephens
, Christopher M. Thomas
, Matthew P. Crump
, Russell J. Cox
, Thomas J. Simpson^*
, Christine L. Willis

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer review

Abstract

Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

Original language	English
Pages (from-to)	5501-5507
Number of pages	7
Journal	Journal of the American Chemical Society
Volume	136
Issue number	14
DOIs	https://doi.org/10.1021/ja501731p
Publication status	Published - 9 Apr 2014
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja501731p

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title = "Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways",

abstract = "Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.",

author = "Gao, \{Shu Shan\} and Joanne Hothersall and Ji'En Wu and Murphy, \{Annabel C.\} and Zhongshu Song and Stephens, \{Elton R.\} and Thomas, \{Christopher M.\} and Crump, \{Matthew P.\} and Cox, \{Russell J.\} and Simpson, \{Thomas J.\} and Willis, \{Christine L.\}",

year = "2014",

month = apr,

day = "9",

doi = "10.1021/ja501731p",

language = "English",

volume = "136",

pages = "5501--5507",

journal = "Journal of the American Chemical Society",

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TY - JOUR

T1 - Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

AU - Gao, Shu Shan

AU - Hothersall, Joanne

AU - Wu, Ji'En

AU - Murphy, Annabel C.

AU - Song, Zhongshu

AU - Stephens, Elton R.

AU - Thomas, Christopher M.

AU - Crump, Matthew P.

AU - Cox, Russell J.

AU - Simpson, Thomas J.

AU - Willis, Christine L.

PY - 2014/4/9

Y1 - 2014/4/9

N2 - Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

AB - Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

UR - https://www.scopus.com/pages/publications/84897981244

U2 - 10.1021/ja501731p

DO - 10.1021/ja501731p

M3 - Article

C2 - 24625190

AN - SCOPUS:84897981244

SN - 0002-7863

VL - 136

SP - 5501

EP - 5507

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 14

ER -

Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Abstract

ASJC Scopus subject areas

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