Comparison of destructively and rinsing gained samples to determine TVC of pig carcasses by bioluminescence

H. D. Werlein

doi:10.1016/S0309-1740(01)00066-3

Comparison of destructively and rinsing gained samples to determine TVC of pig carcasses by bioluminescence

H. D. Werlein^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer review

Abstract

A rinsing-technique with a spraying gun in combination with the ATP-bioluminescence for determining the microbial load was investigated. In each case 3.8-cm² pig-rind was rinsed with buffered peptone water using a modified sprinkle-bottle. The sample-suspensions were gathered in a one-way syringe and sent through a filter cascade consisting of a coarse filter in order to remove bigger particles and a sensitive filter to attach the micro-organisms as well as somatic cells. This procedure was followed by an extraction of the ATP from the sensitive filter with CellSolver FBC-solution. The destructively gained samples were determined on their TVC by the conventional method. Results of the carcass-surface spray test combined with bioluminescence compared to the conventional destructive method followed by plate-count showed a correlation r=0.93 with a detection limit down to 10⁴ cfu/cm².

Original language	English
Pages (from-to)	165-168
Number of pages	4
Journal	Meat science
Volume	59
Issue number	2
E-pub ahead of print	11 Jul 2001
DOIs	https://doi.org/10.1016/S0309-1740(01)00066-3
Publication status	Published - Oct 2001

ASJC Scopus subject areas

Food Science

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10.1016/S0309-1740(01)00066-3

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title = "Comparison of destructively and rinsing gained samples to determine TVC of pig carcasses by bioluminescence",

abstract = "A rinsing-technique with a spraying gun in combination with the ATP-bioluminescence for determining the microbial load was investigated. In each case 3.8-cm2 pig-rind was rinsed with buffered peptone water using a modified sprinkle-bottle. The sample-suspensions were gathered in a one-way syringe and sent through a filter cascade consisting of a coarse filter in order to remove bigger particles and a sensitive filter to attach the micro-organisms as well as somatic cells. This procedure was followed by an extraction of the ATP from the sensitive filter with CellSolver FBC-solution. The destructively gained samples were determined on their TVC by the conventional method. Results of the carcass-surface spray test combined with bioluminescence compared to the conventional destructive method followed by plate-count showed a correlation r=0.93 with a detection limit down to 104 cfu/cm2.",

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AB - A rinsing-technique with a spraying gun in combination with the ATP-bioluminescence for determining the microbial load was investigated. In each case 3.8-cm2 pig-rind was rinsed with buffered peptone water using a modified sprinkle-bottle. The sample-suspensions were gathered in a one-way syringe and sent through a filter cascade consisting of a coarse filter in order to remove bigger particles and a sensitive filter to attach the micro-organisms as well as somatic cells. This procedure was followed by an extraction of the ATP from the sensitive filter with CellSolver FBC-solution. The destructively gained samples were determined on their TVC by the conventional method. Results of the carcass-surface spray test combined with bioluminescence compared to the conventional destructive method followed by plate-count showed a correlation r=0.93 with a detection limit down to 104 cfu/cm2.

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JO - Meat science

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Comparison of destructively and rinsing gained samples to determine TVC of pig carcasses by bioluminescence

Abstract

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