Feruloyl esterases from Schizophyllum commune to treat food industry side-streams

Annabel Nieter; Sebastian Kelle; Diana Linke; Ralf G. Berger

doi:10.1016/j.biortech.2016.08.045

Feruloyl esterases from Schizophyllum commune to treat food industry side-streams

Annabel Nieter^*
, Sebastian Kelle
, Diana Linke
, Ralf G. Berger

^*Corresponding author for this work

Institute of Food Chemistry

Research output: Contribution to journal › Article › Research › peer review

Abstract

Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L⁻¹. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.

Original language	English
Pages (from-to)	38-46
Number of pages	9
Journal	Bioresource technology
Volume	220
DOIs	https://doi.org/10.1016/j.biortech.2016.08.045
Publication status	Published - 12 Aug 2016

UN Sustainable Development Goals (SDGs)

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Agro-industrial side-streams
Basidiomycota
Benzoic acid ester
Feruloyl esterase
Pichia pastoris

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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10.1016/j.biortech.2016.08.045

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title = "Feruloyl esterases from Schizophyllum commune to treat food industry side-streams",

abstract = "Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60\%), ferulic (>80\%) and p-coumaric acid (100\%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.",

keywords = "Agro-industrial side-streams, Basidiomycota, Benzoic acid ester, Feruloyl esterase, Pichia pastoris",

author = "Annabel Nieter and Sebastian Kelle and Diana Linke and Berger, \{Ralf G.\}",

note = "Funding information: Support of the work by the Federal Ministry of Education and Research cluster Biokatalyse2021 (P 37) and by M. Bunzel (Department of Food Chemistry and Phytochemistry, KIT, Karlsruhe, Germany) is gratefully acknowledged.",

year = "2016",

month = aug,

day = "12",

doi = "10.1016/j.biortech.2016.08.045",

language = "English",

volume = "220",

pages = "38--46",

journal = "Bioresource technology",

issn = "0960-8524",

publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - Feruloyl esterases from Schizophyllum commune to treat food industry side-streams

AU - Nieter, Annabel

AU - Kelle, Sebastian

AU - Linke, Diana

AU - Berger, Ralf G.

N1 - Funding information: Support of the work by the Federal Ministry of Education and Research cluster Biokatalyse2021 (P 37) and by M. Bunzel (Department of Food Chemistry and Phytochemistry, KIT, Karlsruhe, Germany) is gratefully acknowledged.

PY - 2016/8/12

Y1 - 2016/8/12

N2 - Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.

AB - Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.

KW - Agro-industrial side-streams

KW - Basidiomycota

KW - Benzoic acid ester

KW - Feruloyl esterase

KW - Pichia pastoris

UR - https://www.scopus.com/pages/publications/84989907860

U2 - 10.1016/j.biortech.2016.08.045

DO - 10.1016/j.biortech.2016.08.045

M3 - Article

C2 - 27566510

AN - SCOPUS:84989907860

SN - 0960-8524

VL - 220

SP - 38

EP - 46

JO - Bioresource technology

JF - Bioresource technology

ER -

Feruloyl esterases from Schizophyllum commune to treat food industry side-streams

Abstract

UN Sustainable Development Goals (SDGs)

Keywords

ASJC Scopus subject areas

Access to Document

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