Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms

Maria Matos; Maria A. Pereira; Pier Parpot; António G. Brito; Regina Nogueira

doi:10.1016/j.chemosphere.2014.06.094

Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms

Maria Matos^*, Maria A. Pereira, Pier Parpot, António G. Brito, Regina Nogueira

^*Corresponding author for this work

Institute of Sanitary Engineering and Waste Management

Research output: Contribution to journal › Article › Research › peer review

Abstract

The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL^-1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.

Original language	English
Pages (from-to)	295-302
Number of pages	8
Journal	CHEMOSPHERE
Volume	117
Issue number	1
Early online date	13 Aug 2014
DOIs	https://doi.org/10.1016/j.chemosphere.2014.06.094
Publication status	Published - Dec 2014

Keywords

Antibiotics
Bacterial community
Nitrifying biofilms
Resistance genes
Tetracycline removal

ASJC Scopus subject areas

Environmental Engineering
Environmental Chemistry
General Chemistry
Pollution
Health, Toxicology and Mutagenesis

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10.1016/j.chemosphere.2014.06.094

https://hdl.handle.net/1822/31643Licence: Unspecified

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title = "Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms",

abstract = "The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL-1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.",

keywords = "Antibiotics, Bacterial community, Nitrifying biofilms, Resistance genes, Tetracycline removal",

author = "Maria Matos and Pereira, {Maria A.} and Pier Parpot and Brito, {Ant{\'o}nio G.} and Regina Nogueira",

note = "Funding Information: The work described in this paper was financially supported by FCT – Foundation for Science and Technology (Portugal) – by the PhD student grant SFRH/BD/44596/2008 . ",

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AU - Parpot, Pier

AU - Brito, António G.

AU - Nogueira, Regina

N1 - Funding Information: The work described in this paper was financially supported by FCT – Foundation for Science and Technology (Portugal) – by the PhD student grant SFRH/BD/44596/2008 .

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N2 - The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL-1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.

AB - The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL-1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.

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KW - Nitrifying biofilms

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Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms

Abstract

Keywords

ASJC Scopus subject areas

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