Metabolic Pathway Rates and Culture Fluorescence in Batch Fermentations of Clostridium Acetobutylicum

Stoichiometric relationships in the metabolic pathway of Clostridium acetobutylicum are used to formulate a framework for the analysis of pathway rates. This analytical tool, alone and in conjunction with intracellular NAD(P)H fluorescence measurements, is applied to two batch fermentations, one acidogenic and one solventogenic, to provide information on intracellular activities not readily obtainable by other methods.