@article{c7344f72f8a747aa96355c011a1a5cf5,
title = "Photosensitive poly-l-lysine/heparin interpolyelectrolyte complexes for delivery of genetic drugs",
abstract = "Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions-such as model oligonucleotide, siRNA, and pDNA-were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.",
keywords = "Heparin, Interpolyelectrolyte complex, PDNA, Photosensitive, Poly-l-lysine, SiRNA",
author = "Viktor Korzhikov-Vlakh and Iuliia Katernuk and Iuliia Pilipenko and Antonina Lavrentieva and Ivan Guryanov and Vladimir Sharoyko and Manshina, {Alina A.} and Tennikova, {Tatiana B.}",
note = "Funding information: The financial support was provided by Russian Science Foundation (project no. 19-73-10045). Ms. I. Katernuk is grateful to the German-Russian Interdisciplinary Science Center (G-RISC) for the supporting one-month internship (project C-2019a-2) at the Institute of Technical Chemistry, Leibniz University Hannover (Hannover, Germany). The authors are grateful to: Arto Urtti and Marika Rupponen, University of Eastern Finland, as well as Thomas Scheper, Institute of Technical Chemistry, Leibniz University for valuable discussions and help. The authors also would like to thank the Magnetic Resonance Research Centre and Centre for Molecular and Cell Technologies of St. Petersburg State University for NMR and TEM investigations, respectively Funding: The financial support was provided by Russian Science Foundation (project no. 19-73-10045). Ms. I. Katernuk is grateful to the German–Russian Interdisciplinary Science Center (G-RISC) for the supporting one-month internship (project C-2019a-2) at the Institute of Technical Chemistry, Leibniz University Hannover (Hannover, Germany).",
year = "2020",
month = may,
day = "8",
doi = "10.3390/POLYM12051077",
language = "English",
volume = "12",
journal = "Polymers",
issn = "2073-4360",
publisher = "Multidisciplinary Digital Publishing Institute",
number = "5",
}