PspA can form large scaffolds in Escherichia coli

Kerstin Standar; Denise Mehner; Hendrik Osadnik; Felix Berthelmann; Gerd Hause; Heinrich Lünsdorf; Thomas Brüser

doi:10.1016/j.febslet.2008.09.002

PspA can form large scaffolds in Escherichia coli

Kerstin Standar, Denise Mehner, Hendrik Osadnik, Felix Berthelmann, Gerd Hause, Heinrich Lünsdorf, Thomas Brüser^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer review

Abstract

The phage shock protein A (PspA) of Escherichia coli stabilizes the cytoplasmic membrane under stress conditions. Here we demonstrate that PspA can form hollow spherical or prolate spheroidal particles of about 30-40 nm diameter with a scaffold-like arrangement of protein subunits at the surface. The 'PspA-scaffold' is the basic structure that is common to all particles. The PspA-scaffold may be of fundamental importance, as it could allow PspA to stabilize the integrity of membranes through numerous contact points over a large surface area.

Original language	English
Pages (from-to)	3585-3589
Number of pages	5
Journal	FEBS Letters
Volume	582
Issue number	25-26
DOIs	https://doi.org/10.1016/j.febslet.2008.09.002
Publication status	Published - 29 Oct 2008
Externally published	Yes

Keywords

Membrane stabilization
Membrane stress
Phage shock protein

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.09.002

Cite this

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title = "PspA can form large scaffolds in Escherichia coli",

abstract = "The phage shock protein A (PspA) of Escherichia coli stabilizes the cytoplasmic membrane under stress conditions. Here we demonstrate that PspA can form hollow spherical or prolate spheroidal particles of about 30-40 nm diameter with a scaffold-like arrangement of protein subunits at the surface. The 'PspA-scaffold' is the basic structure that is common to all particles. The PspA-scaffold may be of fundamental importance, as it could allow PspA to stabilize the integrity of membranes through numerous contact points over a large surface area.",

keywords = "Membrane stabilization, Membrane stress, Phage shock protein",

author = "Kerstin Standar and Denise Mehner and Hendrik Osadnik and Felix Berthelmann and Gerd Hause and Heinrich L{\"u}nsdorf and Thomas Br{\"u}ser",

note = "Funding Information: We are grateful to Jan R. Andreesen and Gary Sawers for discussions and support. This work was supported by the Deutsche Forschungsgemeinschaft with Grant BR2285/2-2 to T.B. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

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AU - Standar, Kerstin

AU - Mehner, Denise

AU - Osadnik, Hendrik

AU - Berthelmann, Felix

AU - Hause, Gerd

AU - Lünsdorf, Heinrich

AU - Brüser, Thomas

N1 - Funding Information: We are grateful to Jan R. Andreesen and Gary Sawers for discussions and support. This work was supported by the Deutsche Forschungsgemeinschaft with Grant BR2285/2-2 to T.B. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2008/10/29

Y1 - 2008/10/29

N2 - The phage shock protein A (PspA) of Escherichia coli stabilizes the cytoplasmic membrane under stress conditions. Here we demonstrate that PspA can form hollow spherical or prolate spheroidal particles of about 30-40 nm diameter with a scaffold-like arrangement of protein subunits at the surface. The 'PspA-scaffold' is the basic structure that is common to all particles. The PspA-scaffold may be of fundamental importance, as it could allow PspA to stabilize the integrity of membranes through numerous contact points over a large surface area.

AB - The phage shock protein A (PspA) of Escherichia coli stabilizes the cytoplasmic membrane under stress conditions. Here we demonstrate that PspA can form hollow spherical or prolate spheroidal particles of about 30-40 nm diameter with a scaffold-like arrangement of protein subunits at the surface. The 'PspA-scaffold' is the basic structure that is common to all particles. The PspA-scaffold may be of fundamental importance, as it could allow PspA to stabilize the integrity of membranes through numerous contact points over a large surface area.

KW - Membrane stabilization

KW - Membrane stress

KW - Phage shock protein

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JO - FEBS Letters

JF - FEBS Letters

IS - 25-26

ER -

PspA can form large scaffolds in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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