Abstract
High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 739-783 |
| Number of pages | 45 |
| Journal | Biotechnology advances |
| Volume | 36 |
| Issue number | 3 |
| E-pub ahead of print | 5 Feb 2018 |
| DOIs | |
| Publication status | Published - May 2018 |
Keywords
- Aspergillus spp.
- Combinatorial biosynthesis
- DNA assembly
- Heterologous expression
- Microbial secondary metabolites
- Pathway engineering
- Promoter Regions, Genetic
- Multigene Family
- Bridged Bicyclo Compounds, Heterocyclic
- Cloning, Molecular/methods
- Computational Biology/methods
- Microorganisms, Genetically-Modified
- Tricarboxylic Acids
- Lovastatin/genetics
- Polyketides/metabolism
- Polymerase Chain Reaction/methods
- Recombination, Genetic
- Genetic Engineering/methods
- Secondary Metabolism/genetics
- Biosynthetic Pathways/genetics
- Aspergillus/genetics
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Bioengineering
- Biotechnology
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