The asparagine synthetase gene VfAS1 is strongly expressed in the nitrogen-fixing zone of broad bean (Vicia faba L.) root nodules

Helge Küster; Ulrike Albus; Martin Frühling; Svetlana A. Tchetkova; Igor A. Tikhonovitch; Alfred Pühler; Andreas M. Perlick

doi:10.1016/S0168-9452(97)04607-4

The asparagine synthetase gene VfAS1 is strongly expressed in the nitrogen-fixing zone of broad bean (Vicia faba L.) root nodules

Helge Küster, Ulrike Albus, Martin Frühling, Svetlana A. Tchetkova, Igor A. Tikhonovitch, Alfred Pühler^*, Andreas M. Perlick

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer review

Abstract

A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.

Original language	English
Pages (from-to)	89-95
Number of pages	7
Journal	Plant science
Volume	124
Issue number	1
DOIs	https://doi.org/10.1016/S0168-9452(97)04607-4
Publication status	Published - 18 Apr 1997
Externally published	Yes

Keywords

Nodule nitrogen metabolism
Nodule-specific cDNA library
Tissue-print hybridization

ASJC Scopus subject areas

Genetics
Agronomy and Crop Science
Plant Science

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10.1016/S0168-9452(97)04607-4

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title = "The asparagine synthetase gene VfAS1 is strongly expressed in the nitrogen-fixing zone of broad bean (Vicia faba L.) root nodules",

abstract = "A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.",

keywords = "Nodule nitrogen metabolism, Nodule-specific cDNA library, Tissue-print hybridization",

author = "Helge K{\"u}ster and Ulrike Albus and Martin Fr{\"u}hling and Tchetkova, {Svetlana A.} and Tikhonovitch, {Igor A.} and Alfred P{\"u}hler and Perlick, {Andreas M.}",

note = "Funding information: S.A. Tchetkova and I.A. Tikhonovitch acknowledge grants from the {\textquoteleft}Bundesministerium fur Ernahrung, Landwirtschaft und Forsten{\textquoteright} (BML).",

year = "1997",

month = apr,

day = "18",

doi = "10.1016/S0168-9452(97)04607-4",

language = "English",

volume = "124",

pages = "89--95",

journal = "Plant science",

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number = "1",

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T1 - The asparagine synthetase gene VfAS1 is strongly expressed in the nitrogen-fixing zone of broad bean (Vicia faba L.) root nodules

AU - Küster, Helge

AU - Albus, Ulrike

AU - Frühling, Martin

AU - Tchetkova, Svetlana A.

AU - Tikhonovitch, Igor A.

AU - Pühler, Alfred

AU - Perlick, Andreas M.

N1 - Funding information: S.A. Tchetkova and I.A. Tikhonovitch acknowledge grants from the ‘Bundesministerium fur Ernahrung, Landwirtschaft und Forsten’ (BML).

PY - 1997/4/18

Y1 - 1997/4/18

N2 - A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.

AB - A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.

KW - Nodule nitrogen metabolism

KW - Nodule-specific cDNA library

KW - Tissue-print hybridization

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U2 - 10.1016/S0168-9452(97)04607-4

DO - 10.1016/S0168-9452(97)04607-4

M3 - Article

AN - SCOPUS:0343416950

SN - 0168-9452

VL - 124

SP - 89

EP - 95

JO - Plant science

JF - Plant science

IS - 1

ER -

The asparagine synthetase gene VfAS1 is strongly expressed in the nitrogen-fixing zone of broad bean (Vicia faba L.) root nodules

Abstract

Keywords

ASJC Scopus subject areas

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